Unigenic evolution: a novel genetic method localizes a putative leucine zipper that mediates dimerization of the Saccharomyces cerevisiae regulator Gcr1p
Article Abstract:
A method called unigenic evolution, a statistical analysis of mutability in evolutionary variants of a single gene, is described. It was applied to map the functionally important domains in Gcr1p of Saccharomyces cerevisiae. Multiple rounds of error-prone PCR mutagenesis on the variants of Gcr1p generated in vitro and in vivo identified four hypomutable regions of GCR1. Analysis of the regions by alternating insertion, deletion and point mutation showed that some of these regions may correspond to functionally important surface domains that are important for Gcr1p homodimerization. In particular, a putative leucine zipper was identified in region D. A diagram of the unigenic evolution of GCR1 is presented.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1995
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Potential RNA binding proteins in Saccharomyces cerevisiae identified as suppressors of temperature-sensitive mutations in NPL3
Article Abstract:
Saccharomyces cerevisiae yeast possess a special gene, NPL3, which is responsible for encoding a protein that performs functions similar to heterogeneous nuclear ribonucleoproteins (hnRNP). NPL3 plays an active role in RNA export, import and rRNA processing. Several suppressors contained in the temperature-sensitive allele of NPL3, npl3-1, were isolated and two suppressors were identified with a newly defined gene, HRP1, responsible for encoding a protein similar to hnRNP A/B proteins of vertebrates. It was discovered that mutations in HRP1 suppress all temperature-sensitive npl3 alleles but do not bypass an npl3 null allele.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1996
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