Conditional mutations in the yeast DNA primase genes affect different aspects of DNA metabolism and interactions in the DNA polymerase alpha-primase complex

Article Abstract:

Saccharomyces cerevisiae conditional mutants with respect to the DNA primases genes, pri1 andpri2, exhibit increased spontaneous mutation and intrachromosomal recombinationrates. This is attributed to the generation of sticky ends resulting from defective replication in the mutants. Sporulation was also compromised in the mutants with unsporulated cells containing one nucleus. This suggests that the mutations have adverse effects on meiosis. Further studies also revealed that the small primase subunit determines enzyme activity while the large subunit mediates interaction between the DNA polymerase alpha and the small subunit.

DNA, Saccharomyces

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MUTS, proofreading and cancer

Article Abstract:

Research done on the mutatorS (mutS) of Escherichia coli has contributed greatly in explaining tumorigenesis. Siegel and Bryson showed in 1967 that most of the mutations caused by mutS were temporary because the majority could be reverted by 2-aminopurine and ethylmethane sulfate. In 1972, Cox, Degnen and Scheppe reported that mutS cause AT -> GC and GC->AT transitions and that these mutations were very similar to the wild type. No other mutator gene in bacteria or yeast had a similar impact in science. What are missing at the moment are studies on mutagenesis, fitness, selection and drift.

Analysis, Gene mutations, Gene mutation, Escherichia coli, Carcinogenesis

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