The effects of nutrient limitation on styrene metabolism in Pseudomonas putida CA-3
Article Abstract:
The ability of Pseudomonas putida CA-3 to degrade styrene and intermediates of styrene was investigated under continuous culture conditions. Oxidation of styrene and the formation of styrene oxide isomerase and phenylacetaldehyde dehydrogenase were used as measures of styrene degradation. Cells exhibited high levels of enzymatic activities when grown on glutamate and succinate despite limited carbon and energy sources. In contrast, cells grown on styrene or phenylacetic acid (PAA) exhibited low levels of enzyme activities, suggesting that styrene or PAA repress the catabolite potential of P. putida CA-3.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Effect of growth rate, nutrient limitation and succinate on expression of TOL pathway enzymes in response to m-xylene in chemostat cultures of Pseudomonas putida (pWWO)
Article Abstract:
The effect of growth conditions on the expression of the TOL pathway in Pseudomonas putida was investigated. The activity of the upper-pathway enzyme BADH and the meta-pathway enzyme C230 in cell-free extracts and the oxygen consumption by washed cells upon addition of m-xylene were quantified. Nutrient limitation when m-xylene is the sole carbon and energy source was cited as a significant factor in the expression level of the upper part of the pathway.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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Indigo formation by microorganisms expressing styrene monooxygenase activity
Article Abstract:
Microorganisms expressing the styrene monooxygenase enzyme can transform indole to indigo. Two strains of Psuedomonas putida were found to express this enzyme and convert indole to indigo. Indole induces enzyme expression of bacteria grown on solid media while styrene induces enzyme expression in both solid and liquid media. Both strains of P. putida were found to yield high-purity indigo from indole.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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