In situ detection of high levels of horizontal plasmid transfer in marine bacterial communities
Article Abstract:
The study investigated the genetic transfer of the conjugation plasmid pBF1 from Pseudomonas putida to bacterial communities in seawater. This was done using green fluorescent protein (GFP) as an in situ detection system. Conjugation experiments performed indicate that plasmids were transferred on surfaces and in bulk seawater. Results also demonstrate the increased sensitivity of the GFP system in detecting conjugations. However, since oxygen is required for the maturation of GFP. This would make the system applicable only in aerobic environments.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Conjugative plasmids isolated from bacteria in marine environments show various degrees of homology to each other and are not closely related to well-characterized plasmids
Article Abstract:
A research was conducted to study the variation and distribution of conjugative plasmids from different marine bacterial communities. Pseudomonas putida was utilized as a model recipient bacterium while bacterial community samples were collected from the shoreline. A representative plasmid from each structural group was evaluated for transfer abilities. Results showed the transfer of Hgr plasmids to the model recipients which occurred in artificial seawater without added nutrients.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Abundance of Tn3, Tn21, and Tn501 transposase (tnpA) sequences in bacterial community DNA from marine environments
Article Abstract:
The abundance of the tnpA transposase genes from the transposons Tn3, Tn21 and Tn501 was measured in total bacterial community DNA samples from marine environments. PCR was used to analyze bulk water and air-water interface samples from rural and coastal sites. Tn21 tnpA was the most abundant, estimated to occur in 1 out of 1,000 to 10,000 bacteria, and could be detected in a total community DNA sample as small as 0.037 ng.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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