Novel methods for genetic transformation of natural Bacillus subtilis isolates used to study the regulation of the mycosubtilin and surfactin synthetases
Article Abstract:
Two novel methods that stimulate the natural and genetic transformation of natural isolates of Bacillus subtilis, which has low genetic competence levels, are described. The methods can be easily used to study the regulation of synthetases of mycosubtilin and surfactin, the two commonly used lipopeptide antibiotics.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
User Contributions:
Comment about this article or add new information about this topic:
Changing a single amino acid in Clostridium perfringens [beta]-toxin affects the efficiency of heterologous secretion by Bacillus subtilis
Article Abstract:
The studies related to differences in the global gene regulation responses of Bacillus subtilis to overproduction of either [beta]-toxin or [beta]-toxoid by transcriptomics showed that the intrinsic properties of a heterologous protein could play a decisive role when optimizing heterologous protein production.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
User Contributions:
Comment about this article or add new information about this topic:
Expression of transcription activator comK of Bacillus subtilis in the heterologous host Lactococcus lactis leads to a genome-wide repression pattern: A case study of horizontal gene transfer
Article Abstract:
A study was conducted on horizontal gene transfer (HGT) by introduction of the competence transcription factor comK of B. subtilis into the recipient L. lactis. It was observed that comK is not functional in L. lactis as it is in B. subtilis.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Factors influencing germination of Bacillus subtilis spores via activation of nutrient receptors by high pressure
- Abstracts: Development and characterization of a subtilin-regulated expression system in Bacillus subtilis: Strict control of gene expression by addition of subtilin
- Abstracts: Sequence analysis of the Bacillus subtilis chromosome region between the serA and kdg loci cloned in a yeast artificial chromosome
- Abstracts: Genetic modification of Bacillus subtilis for production of D-chiro-inositol, an investigational drug candidate for treatment of type 2 diabetes and polycystic ovary syndrome
- Abstracts: Use of a disposable water filter for prevention of false-positive results due to nontuberculosis mycobacteria in a clinical laboratory performing routine acid-fast staining for tuberculosis