Novel pyoverdine biosynthesis gene(s) of Pseudomonas aeruginosa PAO
Article Abstract:
The expression of a plasmid containing the Pseudomonas aeruginosa PAO 15.1 kb chromosomal fragment or its 10.8 kb derivative restores the wild-type yellow-green phenotype to P. aeruginosa pyoverdine-deficient (pvd) mutants. The 10.8 kb fragment is composed of the 66-70 min area of the PAO chromosome and contains genes involved in the synthesis of the pyoverdine chromophore. The pvd mutants produce pseudoverdine that is similar to pyoverdine, but is unable to stimulate the growth of pvd mutants in the presence of iron chelators or to control iron uptake.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Acquisition of iron by the non-siderophore-producing Pseudomonas fragi
Article Abstract:
The Pseudomonas fragi strains unable to produce the iron-sequestering compounds, siderophores, uptake iron by using iron-regulated outer-membrane proteins (IROMP) or siderophores produced by other bacteria. The strains are unable to grow in a medium containing iron chelators or lacking iron. The foreign siderophores stimulate siderophore-controled iron uptake mechanisms and induce growth of the bacteria. The strains are able to use the iron present in their medium probably due to the activity of the IROMPs.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Siderotyping of fluorescent pseudomonads: characterization of pyoverdines of Pseudomonas fluorescens and Pseudomonas putida strains from Antarctica
Article Abstract:
Research was conducted to examine the pyoverdine systems of five independent fluorescent pseudomonad isolates originating from Antarctica. Three different pyoverdine-related siderotypes among the five isolates were detected using pyoverdine-induced growth stimulation, pyoverdine-mediated iron uptake and pyoverdine analysis by isoelectric focusing and electrophoresis. Results provide evidence that siderotyping is a fast way to screen novel pyoverdines.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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