Nucleoid proteins stimulate stringently controlled bacterial promoters: a link between the cAMP-CRP and the (p)ppGpp regulons in Escherichia coli

Article Abstract:

Bacteria lacking the nucleoid-associated protein H-NS and its paralog StpA protein exhibit reduced growth rate but restored by a suppressor mutation in the spoT gene. Data indicate that the decreased stable RNA/protein ratio of the mutant is restored in the suppressor strain suggesting a link between the catabolic repression and a stringent response.

United States, Statistical Data Included, Genetic aspects, Escherichia coli, Genetic regulation, Gene expression, Promoters (Genetics), Genetic transcription, Transcription (Genetics), Chromosomal proteins

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Vesicle-mediated export and assembly of pore-forming oligomers of the enterobacterial ClyA cytotoxin

Article Abstract:

The pore-forming cytotoxin, ClyA protein, from Escherichia coli forms oligomeric pores in the outer-membrane vesicles and expressed constitutively. Vesicle-mediated transport mechanism for ClyA is facilitated by its membrane localization, which helps activation and delivery of the cytotoxic proteins.

Japan, Gram-negative bacteria, Bacterial toxins, Secretion, Biological transport

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Expression of cytotoxicity by potential pathogens in the standard Escherichia coli collection of reference (ECOR) strains

Article Abstract:

Research was conducted to examine cytotoxicity of Escherichia coli, chosen from a reference collection, towards mammalian host cells. The data indicate that production of haemolysin varied with the strains, and those expressing active form of haemolysin strongly affected cells.

Research, Eukaryotic cells, Cells (Biology), Eukaryotes, Cell-mediated cytotoxicity, Cell mediated cytotoxicity, Nucleotide sequence, Base sequence, Intestines, Intestinal microbiology, Toxicity testing, Toxicity tests

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