One-step purification of nisin A by immunoaffinity chromatography
Article Abstract:
A research was conducted to study the purification of lantibiotic nisin A, a ribosomally synthesized and posttranslationally modified antimicrobial peptide, to homogeneity using a single-step immunoaffinity chromatography method. Anti-nisin A monoclonal antibodies were coupled to a HiTrap N-hydroxysuccinimide-activated column from Pharmacia. A reverse-phase chromatography column was utilized to determine the purity of nisin A obtained from the culture supernatant of L. lactis. Results showed that the purification procedure was efficient, specific and reproducible.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Generation of polyclonal antibodies against nisin: immunization strategies and immunoassay development
Article Abstract:
The conjugates of the antimicrobial lanthionine-containing peptide nisin A (nisA) with keyhole limpet hemocyanin (nisA-KLH) induce formation of polyclonal antibodies with high relative affinity and sensitivity in immunized mice. The nisA conjugate with cholera toxin induces antibodies with low affinity and sensitivity. A competitive direct enzyme-linked immunosorbent assay detects five to 100 ng/ml of the antibodies induced by nisA-KLH. The polyclonal antibodies cross-react with nisZ but not with bacteriocins lacking lanthionine.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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PCR detection of the lactocin S structural gene in bacteriocin-producing Lactobacilli from meat
Article Abstract:
The basic molecular biology techniques are suitable to study the strains which are capable of producing bacteriocins. The primer for the PCR amplification of the structural genes of bacteriocins should be selected carefully. Hybridization analysis confirmed the amplification of 75-bp fragment of the gene.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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