Oxidative modification of a Cephalosporin C acylase from Pseudomonas strain N176 and site-directed mutagenesis of the gene
Article Abstract:
Mutation of residue Met-164 to Asn, Gln, Gly, Ser, Pro and Thr improves N176 cephalosporin acylase activity in Pseudomonas N176 strain against glutarylcephalosporanic acid. However, mutation to Leu or Phe causes a reduction in activity. Met-164 is responsible for the decrease in the enzymatic activity by oxidation with hydrogen peroxide. The residue is located in the substrate binding pocket at the active site of the enzyme and its mutation disturbs the enzyme structure.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Insertion-duplication mutagenesis in Streptococcus pneumoniae: targeting fragment length is a critical parameter in use as a random insertion tool
Article Abstract:
A study was conducted to examine the efficiency and specificity of the recombination of insertion plasmids supporting targeting fragments of different lengths. Strain CP1250 was utilized for all pneumococcal transformation reactions while donor DNA samples were quantitated by determining fluorescent intensities in ethidium bromide-stained agarose levels. Results indicated the specificity of targeting insertion-duplication mutagenesis.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
In vivo insertional mutagenesis in Corynebacterium pseudotuberculosis: An efficient means to identity DNA sequences encoding exported proteins
Article Abstract:
Exported proteins of the animal pathogen Corynebacterium pseudotuberculosis is identified using the reporter transposon-based system TnFuZ. Results revealed that thirty-four out of 1,500 mutants had detectable alkaline phosphatase (PhoZ) activity.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: PCR detection of cyclic lipodepsinonapeptide-producing Pseudomonas syringae pv. syringae and similarity of strains
- Abstracts: Isolation and characterization of an attenuated strain of Pseudomonas aeruginosa AC869, a 3,5-dichlorobenzoate degrader
- Abstracts: Identification of an intestinal folate transporter and the molecular basis for hereditary folate malabsorption
- Abstracts: Generation of pluripotent stem cells from neonatal mouse testis. A contextual model for axonal sorting into glomeruli in the mouse olfactory system
- Abstracts: Genetic diversity of Burkholderia solanacearum (synonym Pseudomonas solanacearum) race 3 in Kenya. Enumeration of respiring Pseudomonas spp. in milk within 6 hours by fluorescence in situ hybridization following formazan reduction