Isolation and characterization of an attenuated strain of Pseudomonas aeruginosa AC869, a 3,5-dichlorobenzoate degrader
Article Abstract:
The mouse pathogen Pseudomonas aeruginosa AC869 was employed as a model to determine whether it could be attenuated without affecting its capacity to degrade 3,5-dichlorobenzoate, to grow in common laboratory media and to survive in 3,5-dichlorobenzoate-contaminated soil. A less virulent mutant, AC869-11, was isolated by utilizing bacteriophage E79. AC869-11 produced substantially less O antigen on its LPS than AC869 when grown in mouse lung and cecal mucus. Despite attenuation, AC869-11 grew as well as AC869 with 3,5-dichlorobenzoate as the only carbon and energy source.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Purification and characterization of two bifunctional chitinases/lysozymes extracellularly produced by Pseudomonas aeruginosa K-187 in a shrimp and crab shell powder medium
Article Abstract:
The purification and characterization of two extracellular chitinases, FI and FII from Pseudomonas aeruginosa K-187, in a shrimp and crab medium show that both the enzymes show lysozyme activity, and are activated by copper. The enzymes differ in their optimum pH, optimum temperature, pH stability and thermal stability. They are capable of hydrolyzing chitin and are purified from the culture supernatant of P. aeruginosa K-187. Their molecular weight is determined using gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Transporter-mediated uptake of 2-chloro- and 2-hydroxybenzoate by Pseudomonas huttiensis strain D1
Article Abstract:
The mechanisms of uptake of 2-cholorobenzoate (2-CBA) and 2-hydroxybenzoate (2-HBa) by Pseudomonas huttiensis strain D1 are investigated. The findings suggest that multiple mechanisms for substrate uptake were coupled to the same catabolic enzyme.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
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