Carbon limitation induces sigma(super S)-dependent gene expression in Pseudomonas fluorescens in soil

Article Abstract:

Researchers created a reporter gene for measuring carbon availability in Pseudomonas fluorescens in soil. Carbon limitation causes citrate-repressible beta-galactosidase activity. A double reporter for carbon and nitrogen limitation was also developed.

Health aspects, Gene expression

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Input of protein to lake water microcosms affects expression of proteolytic enzymes and the dynamics of Pseudomonas spp

Article Abstract:

An input of protein to lake water does not cause a pronounced shift in Pseudomonas spp. populations although it does cause an increase in phytoplankton biomass and bacterial abundance. It also causes the production of a protein related to the Pseudomonas fluorescens alkaline proteinase.

Pseudomonas, Proteins, Lake ecology

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Oxygen-sensing reporter strain of Pseudomonas fluorescens for monitoring the distribution of low-oxygen habitats in soil

Article Abstract:

An oxygen-sensitive biosensor was developed based on a transcriptional fusion of a modified anaerobic regulation of arginine deiminase and nitrate reductase pathways-dependent arcDABC promoter of Pseudomonas aeruginosa PAO1 to the lacZ structural gene of Escherichia coli. The P fluorescens biosensor CHA900 was then used to analyze oxygen concentrations in a plant-soil microcosm. Results indicate the utility of the biosensor for detecting low oxygen concentrations in the rhizosphere and other soil habitats.

Design and construction, Genetic aspects, Habitat (Ecology), Escherichia coli, Biosensors, Habitats, Promoters (Genetics)

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