PCR amplification and species determination of microsporidia in formalin-fixed feces after immunomagnetic separation

Article Abstract:

A study was conducted to evaluate methods used to prepare formalin-fixed fecal specimens for PCR amplification of microsporidial DNA. Two methods, one involving immunomagnetic separation to concentrate microsporidial spores from fecal specimens and the other involving the use of Chelex resin to remove inhibitory substances, were employed that allowed PCR detection and subsequent identification of microsporidia. Both techniques led to reproducible, confirmed detection of microsporidia in formalinized fecal specimens.

Research, Usage, Polymerase chain reaction, Protozoa, Protozoans, Microsporidia, Microsporida

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Persistence of category A select agents in the environment

Article Abstract:

The information of the persistence of select agents on the Centers for Disease Control (CDC) category A agent list in the environment and its implications in a terrorism response are discussed. The development of standardized testing methods and conditions for assessing survival is very important and the usual multiphasic die-off rates should be considered to estimate the risk posed by pathogens in the environment.

Analysis, Environmental aspects, Control, Aerosols, Pathogenic microorganisms, Bioterrorism

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Significance of fomites in the spread of respiratory and enteric viral disease

Article Abstract:

The role of fomites in the spread of viral infections is examined. Fomites are identified as potential vehicle for disease transmission and they get contaminated with micro-organisms through direct contact with soiled hands, sneezing and vomiting.

Causes of, Development and progression, Virus diseases, Disease transmission

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