PCR detection of genes encoding nitrite reductase in denitrifying bacteria

Article Abstract:

Two sets of PCR primers have been designed to amplify fragments of the gene coding for nitric reductase, a key enzyme involved in bacterial denitrification. Consensus regions in gene sequences encoding the two forms of nitrite reductase, cd(sub 1)-nir and Cu-nir were used in the preparation of the primers. Results show that the primers were able to amplify the correct gene, although they failed to amplify genes of nondenitrifying strains.

Genetic aspects, Bacteria, Polymerase chain reaction, Nitrogen oxides, Gene amplification, Nitrogen oxide, Genetic code

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Metabolic profiles and genetic diversity of denitrifying communities in activated sludge after addition of methanol or ethanol

Article Abstract:

The effects of adding methanol and ethanol on genetic and metabolic diversity of denitrifying communities in activated sludge were compared using a pilot-scale plant with two parallel lines. The results support the hypotheses that compositions of denitrifier communities change during predenitrification processes when external carbon sources are added, though no severe effect could be seen from an operational point of view.

All Other Basic Organic Chemical Manufacturing, Industrial organic chemicals, not elsewhere classified, Ethanol, Ethyl Alcohol Manufacturing, Methanol, Biological diversity, Biodiversity, Alcohol, Alcohol, Denatured

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Activity and composition of the denitrifying bacterial community respond differently to long-term fertilization

Article Abstract:

The long-term effects of different organic and inorganic fertilizers on activity and composition of the denitrifying and total bacterial communities in arable soil are investigated. The results have shown that the addition of different fertilizers affects both the activity and the composition of the denitrifying communities in arable soil on a lorng-term basis.

Science & research, Agricultural Chemicals, Fertilizer Manufacturing, Fertilizers, Bacterial genetics, Chemical properties

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