Persistence of free plasmid DNA in soil monitored by various methods, including a transformation assay
Article Abstract:
The ability of plasmid DNA to persist in natural soil environments for extended periods of time was assessed. The detection procedures consisted of three DNA hybridization protocols and a transformation assay. The results showed that plasmid molecules are hardly detectable after two to five days, depending on the soil type. Clay soil was able to maintain actively transforming plasmid DNA for longer periods than either loamy sand or silty clay types. The results suggest that plasmid DNA may persist in the soil environment long enough to be transferred to competent recipient cells.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Natural transformation of Acinetobacter calcoaceticus by plasmid DNA adsorbed on sand and groundwater aquifer material
Article Abstract:
The transformation of Acinetobacter calcoaceticus by plasmid DNA was studied. The adsorption of plasmid and chromosomal DNA to nonpurified mineral material sampled from the environment was then examined. The microcosms used consists of chemically pure sand in buffered CaCl2 solution or of material from a groundwater aquifer in groundwater. The results suggest that plasmid and chromosomal DNA on these mineral surfaces may be available for uptake by cells, although with different efficiencies.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Identification of multiple plasmids released from recombinant genomes of Hansenula polymorpha by transformation of Escherichia coli
Article Abstract:
Escherichia coli cells were treated with DNAs from recombinant Hansenula polymorpha strains with chromosomal single or tandem multiple integrations of a pUC18-derived expression. The process led to the formation of E. coli transformants containing plasmids of different size and organization from that of the expression plasmid. Their formation was stimulated by DNA damage.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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