Promoter elements in Drosophila melanogaster revealed by sequence analysis
Article Abstract:
Analysis of Drosophila promoter database of 252 independent promoter entries reveals the presence of significant nucleotide sequence irregularities in the proximal promoter region. This region has a tripartite structure, with initiator at plus or minus 5 bp, TATA at -25/-30 bp and a new group of downstream elements at +20/+30 bp from the RNA start site. The strand-specific downstream elements vary from the initiator and TATA in their length, position, representation and multiple sequences. An extended region with increased GC- and decreased A-content with several binding sites for Drosophila transcription factors constitute the overall sequence organization of the promoter region.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1995
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A short 5'-flanking region mediates glucose repression of amylase gene expression in Drosophila melanogaster
Article Abstract:
Glucose repression of amylase gene expression was studied in Drosophila melanogaster. The Bio-Rad protein assay was used to determine amylase expression. Results showed that cis-acting elements in the amylase promoter caused sufficient glucose-repressible gene expression while ADH repression was variable in glucose and non-glucose fed larvae. Proximal and distal amylase promoters were compared and sequence divergence of as much as 40% was noted. Depression of the amylase gene was controlled by upstream promoters.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1993
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Genetic analysis of the Additional sex combs locus of Drosophila melanogaster
Article Abstract:
Gamma radiation was used to isolate new alleles of the Additional sex combs (Asx) locus of Drosophila melanogaster. The Asx region had a higher mutation rate than expected, hence it is either sensitive to radiation damage or is a large gene. Loss of the Asx gene resulted in segmentation defects and altered patterns of expression of the even-skipped gene, which suggests that Asx is a developmental requisite. Transposon tagging of the Asx locus was performed, allowing molecular studies to be started.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
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