Phosphorus-31 and carbon-13 nuclear magnetic resonance studies of glucose and xylose metabolism in cell suspensions and agarose-immobilized cultures of Pichia stipitis and Saccharomyces cerevisiae

Article Abstract:

The rate of glucose and xylose metabolism is higher in aerobic than in anoxic cell suspensions of Pichia stipitis, while ethanol production is slightly lower. This may account for the higher intracellular pH in P. stipitis in the presence of oxygen. However, Saccharomyces cerevisiae shows identical rates of glucose consumption and ethanol production under both conditions. Agarose-immobilized S. cerevisiae shows an increase in the rate of carbohydrate metabolism, but P. stipitis does not. P-31 and C-13 nuclear magnetic resonance spectroscopy is used to analyze the two species.

Analysis, Observations, Nuclear magnetic resonance spectroscopy, Saccharomyces, Glucose metabolism

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Biodegradation of pentachlorophenol in a continuous anaerobic reactor augmented with Desulfitobacterium frappieri PCP-1

Article Abstract:

Biodegradation of pentachlorophenol (PCP) is discussed as it occurs in a continuous anaerobic reactor with added Desulfitobacterium frappieri PCP-1. The reactor was an upflow sludge bed reactor degrading PCP. To estimate efficiency of augmentation the PCP-1 population in the reactor was enumerated through use of a competitive PCR. Anaerobic degradation of PCP is one process that might benefit from addition of a laboratory strain. The PCP-1 strain seemed to compete well with other microorganisms of the mixed bacterial community.

Canada, Statistical Data Included, Research, Biodegradation, Anaerobic bacteria, Bioreactors, Pentachlorophenol

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