Hyperphosphorylation of Msn2p and Msn4p in response to heat shock and the diauxic shift is inhibited by cAMP in Saccharomyces cerevisiae
Article Abstract:
The Msn2p and Msn4p transcription factors of Saccharomyces cerevisiae are hyperphosphorylated by different stress conditions and are differentially regulated. cAMP reverses the phosphorylation, and the results suggest that PKA and stress-activated kinases control these transcription factors by antagonistic phosphorylation.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2000
User Contributions:
Comment about this article or add new information about this topic:
High cAMP levels antagonize the reprogramming of gene expression that occurs at the diauxic shift in Saccharomyces cerevisiae
Article Abstract:
The effect of cAMP on protein synthesis and, thus, the role of the cAMP pathway in regulating gene expression in Saccharomyces cerevisiae, is determined using two-dimensional gel electrophoresis. Results indicate that cAMP plays a minor role on the protein pattern of cells growing exponentially on glucose, although it interfered with changes in gene expression in yeast cultures. A model that gives the cAMP pathway a role in maintaining gene expression is proposed. Such a model would require a decrease in cAMP for metabolic transitions such as the diauxic phase to occur.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Nuclear localization of the C2H2 zinc finger protein Msn2p is regulated by stress and protein kinase A activity
Article Abstract:
Msn2p and partly redundant factor Msn4p are important regulators of stress-responsive gene expression in Saccharamyces cerevisiae. They are necessary for transcription of various genes coding for proteins with stress-protective functions. The two proteins are Cys2His2 zinc finger proteins. They bind to the stress-response element (STRE) and accumulate in the nucleus if there are conditions of stress. C2H2 zinc finger protein Msn2p's localizing in the nucleus is regulated by two things, protein kinase A activity and stress.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Dioctyl phthalate increases the percentage of unsaturated fatty acids with a concomitant decrease in cellular heat shock sensitivity in the yeast Saccharomyces cerevisiae
- Abstracts: Imposition of crossover interference through the nonrandom distribution of synapsis initiation complexes. Pachytene exit controlled by reversal of Mek1-dependent phosphorylation
- Abstracts: Exposure to cadmium elevates expression of genes in the OxyR and OhrR regulons and induces cross-resistance to peroxide killing treatment in Xanthomonas campestris
- Abstracts: Cell cycle-regulated phosphorylation of p220(super.NPAT) by cyclin E/Cdk2 in Cajal bodies promotes histone gene transcription
- Abstracts: A nonproteolytic function of the proteasome is required for the dissociation of Cdc2 and cyclin B at the end of M phase