Phylogeny and classification of bacteria in the genera Clavibacter and Rathayibacter on the basis of 16S rRNA gene sequence analyses

Article Abstract:

The phylogeny and classification of bacteria in the genera Clavibacter and Rathyobacter on the basis of 16S ribosomal RNA gene sequence analyses were examined to develop a classification system based on 16S recombinant DNA sequence analyses. The study made use of the restriction fragment length polymorphism method to differentiate species and subspecies of Clavibacter and Rathyobacter. Results reveal that groups and subgroups delineated by using the approach and that it consistently coincides with the clustering delineated by phylogenetic analysis.

Bacteria, Recombinant DNA

---

Sequence heterogeneity in the two 16S rRNA genes of Phormium yellow leaf phytoplasma

Article Abstract:

The Phormium yellow leaf phytoplasma contains two copies of the 16S rRNA gene that show sequence heterogeneity in four nucleotides. The restriction enzymes, BpmI and BsrI, can differentiate the sequence heterogeneity. The sequence similarity level between the two 16S rRNA genes in one strain is similar to the level of homology in other phytoplasma strains. The Phormium yellow leaf phytoplasma is a phytopathogenic Mollicute that is related to stolbur and German grapevine yellows phytoplasmas.

Genetic aspects, Observations, Nucleotide sequence, Base sequence, Mycoplasmatales

---

Frequency of formation of chimeric molecules as a consequence of PCR coamplification of 16S rRNA genes from mixed bacterial genomes

Article Abstract:

A research was conducted to study the level of occurrence of chimeric sequences after polymerase chain reaction (PCR) using a model in which 16S rRNA genes were amplified from mixed bacterial genomes. The DNASTAR program was utilized to examine the multiple alignment of sequences and sequence similarities. Results showed that the use of PCR in the study of microbial diversity revealed the existence of several novel organisms that cannot be isolated by culture-dependent techniques.

Methods, Polymerase chain reaction, Genomes, Gene amplification

Similar abstracts:

• Abstracts: Phosphorylation and localization of replication protein A during oogenesis and early embryogenesis of Drosophila melanogaster
• Abstracts: Detection of novel methanotrophs using phylogenetic and functional gene probes after methane enrichment. A new chitosanase gene from a Nocardioides sp. is a third member of glycosyl hydrolase family 46
• Abstracts: Lysogenic bacteriophage M1 from Selenomonas ruminantium: isolation, characterization and DNA sequence analysis of the integration site
• Abstracts: Isolation and characterization of microsatellite loci for use in population genetic analysis in the timber rattlesnake, Crotalus horridus
• Abstracts: Protein translocation in the three domains of life: Variations on a theme

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.