Physical and genetic map of the genome of Staphylococcus carnosus TM300
Article Abstract:
A genome map of Staphylococcus carnosus TM300 was developed by pulsed-field gel electrophoresis of DNA fragments taken after digestion with NotI, SfiI and ApaI. The size of the chromosome was found to be about 2590 kb. The fragments were constructed into a physical map through a combination of complementary techniques including multiple and partial digests of genomic DNA, hybridization with homologous gene probes, and cross-Southern hybridization.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Biosynthesis of triacylglcerol in the filamentous fungus Mucor circinelloides
Article Abstract:
Liquid cultures of Mucor circinelloides were studied to examine lipid metabolism. Major labelled lipids in cultures incubated with (super14C)acetate were triacylgylcerol (TAG), phosphatidylcholine (PC) and phosphatidylethanolamine (PE). Phosphatidic acid metabolism to diacylglycerol and TAG was achieved by an increase in the pH to 8.0. GPATT exhibited an obvious selectivity for palmitoyl-CoA and the LPAAT for oleoyl- and linoleoyl-CoA.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Identification of a second lipase gene, gehD, in staphylococcus epidermidis: comparison of sequence with those of other stphylococcal lipases
Article Abstract:
Results demonstrate that similar to other staphylococcal lipases, gehD lipase from S. epidermidis is a 45 kilodalton protein and its gene does not hybridize with the DNA from other staphylococcal strains. Data further suggest that the enzyme is more related to that of S. aureus species than to each other.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2000
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Physical and genetic map of the genome of Campylobacter upsaliensis. Campylobacter upsaliensis exerts a cytolethal distending toxin effect on HeLa cells and T lymphocytes
- Abstracts: Phylogenetic position of yeastlike endosymbionts of anobiid beetles. Novel group within the kingdom Crenarchaeota from boreal forest soil
- Abstracts: Purification and partial characterization of a tripeptidase from Pediococcus pentosaceus K9.2. Development and characterization of lactose-positive Pediococcus species for milk fermentation
- Abstracts: Cloning, analysis and one-step disruption of the ARG5,6 gene of Candida albicans. A novel multidrug efflux transporter gene of the major facilitator superfamily from Candida albicans (FLU1) conferring resistance to fluconazole
- Abstracts: The systematic sequencing of the Bacillus subtilis genome in Japan. Effects of hydration on molecular mobility in phase-bright Bacillus subtilis spores