The systematic sequencing of the Bacillus subtilis genome in Japan

Article Abstract:

The Japanese genome sequencing project on Bacillus subtilis is composed of molecular biologists and physiologists who conduct research on microbialreplication, genetic transcription, protein synthesis and cell division. Researches for genome project have already identified 535 genes since 1985. Furthermore, the systematic sequencing of the entire Bacillus subtilis genome in Japan began in 1991 after receiving a research grant from the Japanese Ministry of Education, Science, Culture and Sports or Monbusho.

Japan, Genomes, Chromosome mapping, Genetic code, Microbial genetics

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Purification of two Bacillus subtilis proteins which cross-react with antibodies directed against eukaryotic protein kinase C, the His HPr kinase and trigger factor

Article Abstract:

Anti-protein kinase C antibodies were used in the identification and isolation of Bacillus subtilis protein kinases. Purification on the basis of antibody recognition using two-dimensional electrophoresis identified two proteins, namely, 70kDa and 67kDa. The 70kDa band was identified as phosphoenolpyruvate-dependent His HPr kinase, Enzyme I of the phosphotransferase system which is involved in sugar transport. The 67kDa, on the other hand, is involved in protein folding.

Cell cycle, Phosphorylation

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Effects of hydration on molecular mobility in phase-bright Bacillus subtilis spores

Article Abstract:

Research was conducted to examine the effects of dehydration and rehydration on radioactive phosphorous and carbon molecular mobility in dormant Bacillus subtilis spores. Data show that phosphorous mobility is dependent on hydration and is confined to core-specific components whereas carbon is immobilized to the water-insoluble core area.

United Kingdom, Statistical Data Included, Usage, Physiological aspects, Spores (Bacteria), Bacterial spores, Hydration, Rehydration solutions, Nuclear magnetic resonance spectroscopy

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