Physical map of the chromosome of the phytopathogenic bacterium Pseudomonas syringae pv. phaseolicola
Article Abstract:
DNA from Pseudomonas syringae pv. phaseolicola completely digested with different restriction enzymes, PacI and PmeI, produced 15 and 16 fragments, respectively. Its chromosome was found to be about 5.65 Mb in size. The different fragments were linked into a circular chromosome map for both enzymes. Partial digestions of DNA from various Tn5 mutants 'tagging' specific bands were produced and the complete and partial products of the digestion separated and identified. By computing the size of the partial products, the different bands were linked into a physical map.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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Pili of Pseudomonas syringae pathovar syringae enhance initiation of bacterial epiphytic colonization of bean
Article Abstract:
Examination of various phases of epiphytic colonization of bush bean reveals that pili of Pseudomonas syringae pathovar syringae R32 induces colonization upon removing unadsorbed bacteria by rinsing. Wild-type bacteria exhibits significant resistance to displacement by rinsing, while non-piliated mutant bacteria exhibit only partial resistance. A pellicle is formed by piliated cells on the surface of stationary liquid cultures, while pellicle formation is absent in non-piliated mutant strains.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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High frequency of conjugation versus plasmid segregation of RP1 in epiphytic Pseudomonas syringae populations
Article Abstract:
Absence of selection pressure for plasmid causes easy transfer of the broad host-range IncP plasmid RP1 from an added Pseudomonas syringae strain to added intraspecific recipient bacteria and to the resident epiphytic bacterial flora. During high humidity levels, the frequency of conjugation among the added donors and recipients is high. Equilibrium between conjugation and plasmid loss, and the slower development of the plasmid-containing cells, are observed.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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