Production and characterization of anti-DNA-RNA monoclonal antibodies and their application in Listeria detection
Article Abstract:
Two different heteropolymers cDNA-mRNA and phi X174 DNA-RNA heteroduplexes used as antigens helped obtain murine monoclonal antibodies (MAbs) specific for DNA-RNA hybrids, both having similar immunogenicities. A biotinylated rRNA gene probe specific for the genus Listeria was hybridized with rRNA in teh solution phase and the hybrids formed were captured is microtiter plate wells precoated with the purified zoD3 MAb. The probe-target hybrids were traced with a streptavidin-alkaline phosphatase conjugate. This assay was specific for the genus Listeria and highly sensitive, allowing the detection of as little as 2.5 pg of target rRNA.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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A ribosomal DNA fragment of Listeria monocytogenes and its use as a genus-specific probe in an aqueous-phase hybridization assay
Article Abstract:
cDNAs prepared from the total RNA of Listeria monocytogenes ATCC 19118 were used as probes to scren a genomic library of the same strain and four clones having ribosomal DNA fragments were identified, one of which was differentially hybridized with cDNA probes to RNA of Listeria monocytogenes and Kurthia zopfii resulting in an HpaII fragment of 0.8 kb that was specific for the Listeria monocytogeness strain. An assay named the heteroduplex nucleic acid (HNA) enzyme-linked immunosorbent assay was also used as a probe is an assay. An enzyme conjugate of streptavidin is detected with captured HNA molecules.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Influence of ammonium salts and cane molasses on growth of Alcaligenes eutrophus and production of polyhydroxybutyrate
Article Abstract:
Analysis of the growth of Alcaligenes eutrophus DSM 545 and production of polyhydroxybutyrate (PHB) as a function of various ammonium substrates reveals that ammonium sulfate is the best stimulator of growth and PHB production. Examination of the influence of cane molasses on the growth and PHB production reveals that growth and production is maximum with 0.3% molasses. Measurement of dry cell weight helps follow the growth and gas chromatography helps determine the PHB levels.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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- Abstracts: Production and characterization of monoclonal antibodies against vegetative cells of Bacillus cereus
- Abstracts: Production of monoclonal antibodies to Listeria monocytogenes and their application to determine the virulence of isolates from channel catfish
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