Production of active chimeric pediocin AcH in Escherichia coli in the absence of processing and secretion genes from the Pediococcus pap operon
Article Abstract:
A study was conducted to examine the minimum requirements for the synthesis and secretion of pediocin AcH in Escherichia coli. Results indicated that the functional mature domain of the Pediococcus antimicrobial peptide is aimed at the E.coli sec machinery and secreted to the periplasm in active form when mingled in frame to the COOH terminus of the secretory protein maltose-binding protein (MBP). This MBP fusion-periplasmic leaky expression system should prove beneficial for production and screening of bioactive peptide activity.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Isolation and characterization of pediocin AcH chimeric protein mutants with altered bactericidal activity
Article Abstract:
A study was conducted to examine the structure and mode of action of pediocin AcH using a collection of pediocin AcH substitution mutants that support altered bacteridical activity. Protein mutants were determined by cloning mutagenized DNA into an Escherichia coli malE plasmid while polymerase chain reaction random mutagenesis was carried out to introduce base substitutions into the papA gene. Results indicated that pediocin AcH cysteines are critical for bacteridical activity.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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The pediocin AcH precursor is biologically active
Article Abstract:
Researchers have revealed that the pediocin AcH precursor is as biologically active as its mature species and has been found to be equally susceptible to protease degradation and disulfide bond reduction. Originally, it has been thought that prebacteriocins produced by gram-positive bacteria were biologically inactive. This was due to the protection of leader peptides on producer cells which lead to the inactivity of precursor molecules.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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