Production of manganic chelates by laccase from the lignin-degrading fungus Trametes (Coriolus) versicolor

Article Abstract:

Laccase extracted from Trametes (Coriolus) versicolor was examined for its ability to oxidize manganous ion to manganic state. The enzyme was capable of oxidizing manganous ion only in the presence of pyrophosphate, phenol and hydrogen peroxide. Other factors, like manganese chelators, pH, presence of oxygen and iron were also important in the oxidative activity of laccase. Based on the results, the only difference found between laccase and manganese peroxidases is the latter's ability to oxidize manganous ion in the absence of a phenolic intermediate.

Physiological aspects, Wood-decaying fungi

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Phenolic azo dye oxidation by laccase from Pyricularia oryzae

Article Abstract:

Laccase from Pyricularia oryzae oxidizes phenolic azo dyes. 2-Methyl-, 2-methoxy-, 2,3-dimethyl-, 2,6-dimethyl-, 2,3-dimethoxy- and 2,6-dimethoxy-substituted 4-(4'-sulfophenylazo)-phenol act as substrates for oxidation. The phenol oxidase, laccase, oxidizes 2,6-dimethyl derivative of 4-(4'-sulfophenylazo)-phenol to 4-sulfophenylhydroperoxide (SPH) and 2,6-dimethyl-1,4-benzoquinone. Oxidation of other substrates leads to the respective benzoquinone and SPH. The proposed mechanism for the formation of benzoquinone and SPH is discussed.

Oxidation-reduction reaction, Oxidation-reduction reactions, Azo compounds

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Cellobiose dehydrogenase from Phanerochaete chrysosporium is encoded by two allelic variants

Article Abstract:

Two allelic variants, cdh-1 and cdh-2, from Phanerochaete chrysosporium encode cellobiose dehydrogenase. The nucleotide sequences of the two alleles exhibit 97% similarity and 86 point mutations between them. Both cdh-1 and cdh-2 have 14 exons and all the introns are present at similar positions. The translation products of these alleles have same amino acid sequences. The restriction fragment length polymorphism analyses of homokaryotic derivatives give the segregation of the CDH alleles.

Genetic aspects, Nucleotide sequence, Base sequence, Fungi, Dehydrogenases, Oxidoreductases, Allelomorphism, Alleles

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