Production of monoclonal antibodies specific for the i and 1,2 flagellar antigens of Salmonella typhimurium and characterization of their respective epitopes
Article Abstract:
A study was conducted to analyze the production of monoclonal antibodies (MAb) for the flagellar antigens of Salmonella typhimurium and to characterize their epitopes. MAb-producing hybridoma cell lines were determined from mice immunized with the purified flagellin while serotyping of isolates of different biphasic Salmonella serotypes were carried out to obtain the specificities of the MAbs for their respective antigens. Results indicated that all H-specific MAbs and commercial typing sera support the same epitope on their corresponding flagellin.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Induction of acid resistance of Salmonella typhimurium by exposure to short-chain fatty acids
Article Abstract:
Research was conducted to examine the induction of acid resistance of Salmonella typhimurium by exposure to short-chain fatty acids (SCFA). To gain insight into the potential role of SCFA in the pathogenesis of S. typhimurium, the effects of SCFA on the acid resistance of the organism were analyzed. Results indicate that SCFA in the gastrointestinal tract of a host animal or in food materials may contribute to the improvement of the virulence of S. typhimurium by increasing acid resistance.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Mechanism by which gamma irradiation increases the sensitivity of Salmonella typhimurium ATCC 14028 to heat
Article Abstract:
DNA and cellular membrane damage measurements are performed to identify the mechanisms by which gamma irradiation and heating increases the sensitivity of Salmonella typhimurium to heat. Results indicate that the degree of irradiation-enhanced heat sensitivity in S. typhimurium is dependent on the association between DNA and the cytoplasmic membrane at multiple sites. The data also suggest that heating could alter DNA polymerase and topoisomerase I.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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