Purification and characterization of an extracellular beta-1,4-mannanase from a marine bacterium, Vibrio sp. strain MA-138
Article Abstract:
Beta-1,4-mannanase isolated from the marine bacterium Vibrio sp. strain MA-138 is an extracellular glycoprotein with a molecular mass of 49 kDa and has maximum activity at pH 6.5 and 40 degree celsius. Polyacrylamide gel electrophoresis shows that beta-1,4-mannanase is a monomer and forms oligosaccharides of different sizes on hydrolysis. Physicochemical and enzymatic properties of the enzyme are comparable to other microbial mannanases.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Cloning of the novel gene encoding beta-agarase C from a marine bacterium, Vibrio sp. strain PO-303, and characterization of the gene product
Article Abstract:
The cloning and expression of the beta-agarase C gene (agaC) from the marine bacterium Vibrio sp. strain PO-303 is described, and the recombinant enzyme (rAgaC) is characterized. The gene beta-agarase C is the first beta-agarase that cannot hydrolyze neoagarooctaose and smaller neoagarooligosaccharides and is hence believed to belong to a novel glycoside hydrolase family.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Cloning, sequencing, and expression in Escherichia coli of the new gene encoding beta-1,3-xylanase from a marine bacterium, Vibrio sp. strain XY-214
Article Abstract:
The beta-1,3-xylanase gene of Vibrio sp. strain XY-214 codes a protein of 460 amino acids with a molecular mass of 51,323 Da, according to researchers who expressed the gene in E. coli. The enzyme hydrolyzes beta-1,3-xylan, producing several xylooligosaccharides.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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