Rapid detection, identification, and enumeration of Escherichia coli cells in municipal water by chemiluminescent in situ hybridization
Article Abstract:
A new, one-day method is presented for chemiluminescent in situ hybridization for detecting, identifying and enumerating Escherichia coli cells in municipal water supplies. The method uses soybean peroxidase-labeled peptide nucleic acid (PNA) probes with a 47-mm diameter membrane filter, providing additional specificity and sensitivity while allowing the use of conventional culturing and filtration techniques.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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Molecular detection and identification of Brettanomyces/Dekkera bruxellensis and Brettanomyces/Dekkera anomalus in spoiled wines
Article Abstract:
The development of a PCR-restriction enzyme (RE) analysis protocol to detect and identify, B. bruxellensis and B. anomalus, is described. Other molecular techniques, such as PCR-DGGE, reverse transcription (RT)-PCR-DGGE, and hybridization with a B.bruxellensis-specific probe, are used as confirmatory tests for the detection of spoilage yeasts in wine samples.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Identification of Dekkera bruxellensis (Brettanomyces) from wine by fluorescence in situ hybridization using peptide nucleic acid probes
Article Abstract:
Researchers describe a peptide nucleic acid probe for the 26S ribosomal DNA of Dekkera bruxellensis that can identify this yeast with 100% sensitivity. This organism is responsible for wine spoilage and is known in the wine industry as Brettanomyces.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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- Abstracts: Comparison of vertical distribution of prokaryotic assemblages in the anoxic Cariacio Basin and Black Sea by use of fluorescence in situ hybridization
- Abstracts: Community analysis of the bacterial assemblages in the winter cover and pelagic layers of a high mountain lake by in situ hybridization
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