Purification and characterization of the Bacillus subtilis levanase produced in Escherichia coli
Article Abstract:
The enzyme levanase produced by Bacillus subtilis is a beta-D-fructofuranosidase which hydrolyzes levan, inulin and sucrose. The molecular mass of the enzyme has been estimated to be 75,000 Da and it is most active at 5.5 pH and 55 degrees centigrade. The levanase breaks down inulin by the single-chain mechanism and a sulfhydral group is involved because in the presence of metal ions such as Ag+ and Hg2+, the enzyme does not breakdown any inulin.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
Isolation of methyl parathion-degrading strain M6 and cloning of the methyl parathion hydrolase gene
Article Abstract:
Researchers have isolated an organophosphate hydrolase from Plesiomonas sp. strain M6 that can convert methyl parathion to para-nitrophenol. The gene for this enzyme could be expressed in Escherichia coli and used to biodegrade organophosphate pesticides such as parathion, methyl parathion, and methamidophos.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
Purification and properties of an enzyme capable of degrading the sheath of Sphaerotilus natans
Article Abstract:
Researchers have discovered a bacterium which produces an enzyme that can degrade the sheath surrounding the bacterium Sphaerotilus natans. This enzyme could be useful in analyzing the structure of the sheath.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Purification and characterization of alkaline xylanases from Bacillus polymyxa. Relative contributions of bacteria, protozoa, and fungi to in vitro degradation of orchard grass cell walls and their interactions
- Abstracts: Structural and kinetic properties of nonglycosylated recombinant Penicillium amagasakiense glucose oxidase expressed in Escherichia coli
- Abstracts: Genetic diversity through the looking glass: effect of enrichment bias. Genetic diversity and dynamics of Sinorhizobium meliloti populations nodulating different alfalfa cultivars in Italian soils
- Abstracts: Polyunsaturated fatty acid biosynthesis in Saccharomyces cerevisiae: expression of ethanol tolerance and the FAD2 gene from Arabidopsis thaliana
- Abstracts: A model for the evolution of high frequencies of males in an androdioecious plant based on a cross-compatibility advantage of males