Purification and characterization of the soluble methane monooxygenase of the type II methanotrophic bacterium Methylocystis sp. strain WI 14
Article Abstract:
The expression, purification and characterization of the enzyme methane monooxygenase (sMMO) and the complete 16S rDNA and sMMO sequencing of isolate WI14 were studied. The sMMO revealed a very narrow substrate specificity and oxidizes some aliphatics, aromatics and halogenated compounds only to their corresponding hydroxylated products. The 16S rDNA sequencing and comparison with that of other known methanotrophic bacteria confirmed that strain WI 14 is very close to the genus Methylocystis.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Kinetics of inhibition of methane oxidation by nitrate, nitrite, and ammonium in a humisol
Article Abstract:
Studies of the kinetics of methane oxidation in soil in a humisol revealed competitive inhibition by ammonium that was modified by further inhibition by the nitrite produced by ammonium oxidation. As a simple competitive inhibitor, ammonium had a Ki of 8 micromolar. Inhibition by nitrite was greater at lower methane concentrations. Nitrate acted as a noncompetitive inhibitor only at greater than 10mM concentrations equivalent to the NaCl concentrations that inhibit methane oxidation.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Nitrous oxide production and methane oxidation by difference ammonia-oxidizing bacteria
Article Abstract:
Ammonia-oxidizing bacteria (AOB) do not contribute to the oxidation and methane synthesis in soils, as proven by a comparative analysis involving six AOB strains. By exposing six different strains of AOB in a HEPES-buffered medium with 7.5 initial pH, it was shown that all cultures generated relatively the same levels of nitrogen oxide. Slight differences in the NO2 production levels of Nitrosospira strains were also observed, due to changes in acidity levels and substrate supplies.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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