Quantitative selective PCR of 16S ribosomal DNA correlates well with selective agar plating in describing population dynamics of indigenous Pseudomonas spp. in soil hot spots
Article Abstract:
A study was conducted to evaluate the reproducibility and suitability of of competitive polymerase chain reaction (PCR) in soil ecosystems for characterizing population dynamics of indigenous Pseudomonas populations in soil hot spots. Results indicate that competitive PCR accurately quantifies DNA in soil reproducibly even when differences between DNA content and the number of culturable bacteria is only about one order of magnitude.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Succession of indigenous Pseudomonas spp. and actinomyces on barley roots affected by the antagonistic strain Pseudomonas fluorescens DR54 and the fungicide imazalil
Article Abstract:
PCR microbiological assays can be used to study the effect of biological control agents on normal microbial populations. This was the conclusion of researchers who compared PCR to culture methods for determining the effect of Pseudomonas fluorescens DR54 and the fungicide imazalil on microbial populations on barley roots.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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Abundance and diversity of Archaea in heavy-metal-contaminated soils
Article Abstract:
Heavy-metal contamination of soil has a detrimental effect on Archaea. This bacteria was thought to exist only in extreme environments, but has been found in marine, freshwater, and terrestrial habitats. 16S rRNA sequence analysis shows it belongs to the Crenarchaeota kingdom.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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