Real-time monitoring of Escherichia coli O157:H7 adherence to beef carcass surface tissues with a bioluminiscent reporter

Article Abstract:

A method using a bioluminescent reporter strain of Escherichia coli that was developed by transformation with plasmid pCGLS1 was proposed to become an alternative means of examining bacteria that are attached to beef carcass surface tissues. The proposed method eliminates the need for obtaining, excising, homogenizing and culturing samples from the tissue surface. In addition, it provides better understanding of the relationship of potential human food-borne pathogens and food animal tissue surfaces. This method was proven useful in studying microbial contamination of beef carcass tissues.

Usage, Beef cattle, Escherichia coli, Bacterial growth, Pathogenic microorganisms, Microbial contamination, Bioluminescence, Cattle carcasses

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Production of bacteriocin inibitory to Listeria species by Enterococcus hirae

Article Abstract:

A strain of Enterococcus hirae was isolated from bovine intestine was further characterized. The bacterium was able to produce a bacteriocin inhibitory to Listeria monocytogenes as well as other Listeria species. The bacteriocin, designated hiraecin S, was inactivated by pronase and papain, but was resistant to catalase. Its antimicrobial effects were highly selective for Listeria spp., with no inhibitory effects observed on a number of Gram-negative and Gram-positive bacteria tested. However, other enterococci were sensitive to hiraecin S.

Antibacterial agents, Identification and classification, Control, Listeria monocytogenes

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Hydroxyapatite adherence as a means to concentrate bacteria

Article Abstract:

A bacterial concentration approach is proposed for rapid microbial detection in foods. The simple and fast hydroxyapatite adherence technique was found to be effective in concentrating, quantitatively and in a viable state, dilute and other micoorganisms. Results also indicate that the approach can detect potential total bacteria concentrations in foods where several species of bacteria may be found.

Food, Bacteria, Food microbiology, Bacteriology, Hydroxylapatite, Hydroxyapatite

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