Sequence and expression analyses of Cytophaga-like hydrolases in a Western Arctic metagenomic library and the Sargasso Sea

Article Abstract:

The investigation of the genes in the Sargasso Sea Whole Genome Sequence (EGS) data set encoding hydrolases potentially used by marine Cytophaga-like bacteria for degrading biopolymers in high-molecular-weight dissolved organic matter (DOM) is presented. The abundance of peptidase genes in the Cytophaga-like fosmid clone provides evidence for the importance of Cyrophaga-like bacteria in the degradation of protein in high-molecular-weight DOM.

United States, Science & research, Research, Genetic aspects, Enzymes, Bacterial genetics, Hydrolases

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Assimilation of polysaccharides and glucose by major bacterial groups in the Delaware Estuary

Article Abstract:

Microautoradiography and fluorescence in situ hybridization are used to assess the contribution of major bacterial groups to the assimilation of extracellular polymeric substances (EPS) and glucose in the Delaware Estuary. The results have revealed that the bacterial community in the Delaware Estuary is not controlled solely by bottom-up factors such as dissolved organic matter.

All Other Basic Organic Chemical Manufacturing, Industrial organic chemicals, not elsewhere classified, Polysaccharides, Analysis, Microbiological research, Glucose, Dextrose, Assimilative capacity (Ecology)

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Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescence in situ hybridization

Article Abstract:

Fluorescence in situ hybridization (FISH) gives very different results than clone libraries of 16S rRNA genes when determining the composition of bacterioplankton communities in coastal waters. Compared to FISH results, alpha-proteobacteria were overrepresented in clone libraries and the Cytophaga-Flavobacter group was underrepresented.

Evaluation, Measurement, In situ hybridization, Marine plankton

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