Repair and enterotoxin synthesis by Staphylococcus aureus after thermal shock
Article Abstract:
The effects of four liquid repair media (1% powdered skim milk in distilled water (PSM), complex medium, M9 minimal salt medium and saline solution) on the repair and enterotoxin production by four heat-shocked Staphylococcus aureus strains (FRI-100, FRI-137, FRI-472 and S6) were studied. Salt tolerance was used as a measure of the repair of damaged cells. PSM was the most efficient medium for promoting the repair of stressed cells. Enterotoxins were observed in all strains after 10 h.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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In vitro assay of Staphylococcus aureus enterotoxin A activity in food
Article Abstract:
An alternative assay for Staphylococcus aureus enterotoxin A (SEA) activity in food, utilizing T-cell proliferation, was developed. Human and rat lymphocytes proliferate in response to concentrations of SEA as low as 1 pg/ml, well below the pathogenic dose of 100 ng. This proliferation assay is highly sensitive, quantitative and simple. Results showed that an in vitro cell proliferation assay is an advantageous substitute for current animal assays for measuring SEA activity in food.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Immunoquantitative real-time PCR for detection and quantification of Staphylococcus aureus enterotoxin B in foods
Article Abstract:
A real-time immunoqunatitative PCR (iqPCR) method for detection of Staphylococcus aureus enterotoxin B (SEB) was developed and evaluated using both pure cultures and foods. IqPCR was not inhibited by any of the foods tested and was able to detect SEB present in these foods and no cross reactivity with SE other than SEB was observed.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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