Retention of enteropathogenicity by viable but nonculturable Escherichia coli exposed to seawater and sunlight
Article Abstract:
Enterotoxigenic strains of Escherichia coli H10407 retain pathogenicity even after being exposed to sunlight, and entering the viable but nonculturable state. When exposed to sunlight, the direct counts of viable cells fail to change. Escherichia coli cells are culturable in the absence of sunlight only when exposed to seawater. Ganglioside-enzyme-linked immunosorbent assay (GM1-ELISA) shows that Escherichia coli continues to produce enterotoxin after exposure to sunlight. GM1-ELISA is found to be superior to the conventional rabbit intestinal loop assay.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Molecular epidemiological survey of rotaviruses in sewage by reverse transcriptase semitested PCR and restriction fragment length polymorphism assay
Article Abstract:
Southern blot hybridization combined with reverse transcription and polymerase chain reaction amplification of the VP7 gene was utilized to detect rotavirus contamination in raw sewage and treated effluent samples. Restriction fragment length polymorphism (RFLP) of the amplification products indicated the presence of a variety of rotavirus dsRNAs belonging to human and animal rotavirus sequences. Furthermore, the treated effluent samples yielded ten different RFLPs of human and animal origin.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Recovery in embryonated eggs of viable but nonculturable Campylobacter jejuni cells and maintenance of ability to adhere to HeLa cells after resuscitation
Article Abstract:
Research conducted on three human isolates of pathogenic bacteria Campylobacter jejuni to investigate their ability to recover culturability after egg passage is analyzed. Results demonstrate that viable but not culturable C. jejuni cells maintain adhesion potential after the passage.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Responses of methanotrophic activity in soils and cultures to water stress. Methanol promotes atmospheric methane oxidation by methanotrophic cultures and soils
- Abstracts: Development and field performance of a broad-spectrum nonviable asporogenic recombinant strain of Bacillus thuringiensis with greater potency and UV resistance
- Abstracts: Flux distributions in anaerobic, glucose-limited continuous cultures of Saccharomyces cerevisiae. Physiological effects of nitrogen starvation in an anaerobic batch culture of Saccharomyces cerevisiae
- Abstracts: Genetic organization of the mle locus and identification of a mleR-like gene from Leuconostoc oenos. Molecular characterization of the gene encoding an 18-kilodalton small heat shock protein associated with the membrane of Leuconostoc oenos
- Abstracts: Analysis of mechanisms regulating expression of the ver-1 gene involved in aflatoxin biosynthesis