Role of N-gamma-acetyldiaminobutyrate as an enzyme stabilizer and an intermediate in the biosynthesis of hydroxyectoine

Article Abstract:

Research was conducted to examine the role of N-gamma-acetyldiaminobutyrate (NADA) as an enzyme stabilizer and an intermediate in the biosynthesis of hydroxyectoine. NADA was purified from mutant strain CHR63 and demonstrated to stabilize the model enzyme rabbit muscle lactate dehydrogenase against inactivation by heat. Results also provide evidence for the hypothesis that NADA is a branch point in the synthesis of the compatible solutes ectoine and hydroxyectoine.

Research, Enzymes, Bacteria, Fluid-electrolyte balance, Osmoregulation, Enzyme regulation

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Application of 13/C nuclear magnetic resonance to elucidate the unexpected biosynthesis of erythritol by Leuconostoc oenos

Article Abstract:

An investigation by 13/C nuclear magnetic resonance analysis on the metabolism of glucose by nongrowing cells of Leuconostoc oenos GM revealed the production of erythritol and glycerol. Elucidation of the pathway of erythritol production using 13/C-labelled glucose showed that the synthesis resulted from the reduction and dephosphorylation of the C-4 moiety of fructose-6-phosphate. Different conditions of aeration affected the metabolism of glucose.

Usage, Physiological aspects, Nuclear magnetic resonance, Bacteriology Cultures and culture media

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Compatible solutes of the hyperthermophile Palaeococcus ferrophilus: Osmoadaptation and thermoadaptation in the order Thermococcales

Article Abstract:

The effect of salinity and growth temperature on the accumulation of intracellular organic solutes is determined in the hyperthermophilic archeon Palaeococcus ferrophilus. The osmotic and heat stress responses of Palaeococcus ferrophilus in regard to compatible solute accumulation are described, the genes for the synthesis of mannosylglycerate (MG) are identified and the recombinant proteins are characterized.

Escherichia coli, Glutamate, Phosphatases, Chemical properties

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