Saccharomyces cerevisiae signature genes for predicting nitrogen deficiency during alcoholic fermentation
Article Abstract:
Genome-wide analysis of the wine yeast strain Saccharomyces cerevisiae PYCC4072 identified 36 genes are highly expressed under conditions of low or absent nitrogen in comparison with a nitrogen-replete condition. A designed DNA chip incorporating some of the genes identified in this study can be developed to assist the winemaker in assessing the nitrogen status of the fermenting grape juice.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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Selection of unusual actinomycetal primary sigma70 factors by plant-colonizing Frankia strains
Article Abstract:
Sigma70 play an important role in gene expression and screening of Frankia strain Eal-12 gene library and sigma70 DNA probing helped detect and characterize a unique actino-mycetal primary sigma70 factor. Genes were transcribed using Frankia strain and the molecular phylogeny of the sigma70 family was obtained with Frankia sequences.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Mechanistic approach to the problem of hybridization efficiency in fluorescent in situ hybridization
Article Abstract:
A model of fluorescent in situ hybridization (FISH) based on the thermodynamics of nucleic acid hybridization is developed. The efficiency of hybridization between the DNA probe and the rRNA is related to the accessibility of the rRNA in FISH when ribosome content and cell permeability are not limiting.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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