Sensitive detection of a novel class of toluene-degrading denitrifiers, Azoarcus tolulyticus, with small-subunit rRNA primers and probes
Article Abstract:
16S rRNA gene-based phylogenetic probes were designed to detect Azoarcus tolulyticus, which are toluene-degrading denitrifiers. Two sets of specific PCR amplification primers and two oligonucleotide hybridization probes were developed and tested against both closely and distantly related environmental isolates. All of these primers and probes were specific to the species of A. tolulyticus. The sensitivity of the PCR amplification primer sets was evaluated with DNA isolated from A. tolulyticus Tol-4 pure culture and from sterile soils seeded with a known number of Tol-4 and Escherichia coli cells.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Microbial diversity and heterogeneity in sandy subsurface soils
Article Abstract:
The hypothesis that spatial isolation and resource heterogeneity are key mechanisms controlling microbial community diversity and structure is tested. The phylogenetic diversity of the microbial communities of five sandy subsurface soils from two geographically separated locations (Delaware and Virginia) is examined.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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