Staphylococcus aureus but not Staphylococcus epidermidis can acquire iron from transferrin

Article Abstract:

A study of the uptake of human transferrin-bound iron by Staphylococci through uptake assays of 55Fe containing-labeled transferrin on cells that are grown under iron-limited and iron-abundant conditions reveals that radioactive iron is taken up by growing cultures of Staphylococcus aureus NCTC 8532 at the mid-to-late exponential phase of growth. Direct contact between the labeled transferrin and the cell is not essential for this process, which is iron-modulated. This growth phase also exhibits siderophore formation. There is no uptake of transferrin-bound iron in Staphylococcus epidermidis ATCC 14990, which does not exhibit protease-, reductase- or siderophore formation.

Physiological aspects, Transferrin, Iron, Iron (Metal)

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A homologue to the Escherichia coli alkyl hydroperoxide reductase AhpC is induced by osmotic upshock in Staphylococcus aureus

Article Abstract:

Staphylococcus aureus induces the expression of four major proteins under extreme osmotic stress caused by sucrose and NaCl. The gene encoding of one of these proteins by PCR method shows that the derived protein is a analog of the Escherichia coli AhpC component of an alkyl hydroperoxide reductase. The induction of AhpC in reaction to stress initiated by osmoregulation is mediated by OxyR.

Fluid-electrolyte balance, Osmoregulation, Amino acid sequence, Amino acid sequencing

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Identification of Staphylococcus aureus genes expressed during growth in milk: a useful model for selection of genes important in bovine mastitis

Article Abstract:

Results demonstrate that an in vitro approach testing for the ability of Staphylococcus aureus to grow on milk provides a fast and simple method to select bovine mastitis pathogenicity genes. Data also indicate that the method is useful in screening growth-inhibitory compounds of bovine mastitis.

Netherlands, Statistical Data Included, Product information, Genetic aspects, Dairy industry, Dairy products industry, Gene expression, Mastitis

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