Staphylokinase as a plasminogen activator component in recombinant fusion proteins
Article Abstract:
Research was conducted to evaluate the use of staphylokinase (SAK) as a plasminogen activator component in recombinant fusion proteins. Both N- and C-terminal fusions to SAK were developed using hirudin as a fusion partner. Recombinant fusion proteins were then secreted from Bacillus subtilis and purified from culture supernatants. Results indicate that C-terminal fusions represent stable configurations for constructing improved thrombolytic agents based on SAK as the plasminogen activator component.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Multiple microfermentor battery: A versatile tool for use with automated parallel cultures of microorganisms producing recombinant proteins and for optimization of cultivation protocols
Article Abstract:
The design and basic operation of a multiple microfermentor battery comprising of eight miniature bioreactors whose culture parameters could be controlled independently is described. It is found that this microfermentor is well suited for automated parallel cultures and process optimization, such as that needed for structural genomics projects.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Compatible-solute-supported periplasmic expression of functional recombinant proteins under stress conditions
Article Abstract:
Researchers describe a technique for producing recombinant proteins that can be done at high salt concentrations. It involves using compatible solutes that not only protect the bacterium but also produce a periplasmic microenvironment to generate high levels of correctly folded recombinant proteins.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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