Stereospecific preparation of an excitatory amino acid antagonist with D-hydantoinase from Agrobacterium tumefaciens as a biocatalyst
Article Abstract:
The Agrobacterium tumefaciens enzyme D-hydantoinase effectively catalyzes the formation of (2r,4R,5S)-2-amino-4,5-(1,2-cyclohexyl)-7-phosphonoheptanoic acid (ACPA). The hydantoinase converts a racemic hydantoin to a stereospecific carbamyl D-amino acid derivative which is converted to ACPA. The enzyme is recovered at the end of the reaction and re-used. The reaction is carried out at 9 pH and 40-45 degrees centigrade. ACPA is a excitatory amino acid antagonist which controls the activity of the mammalian central nervous system amino acid receptors.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Preparation of GM1 ganglioside with sialidase-producing marine bacteria as a microbial biocatalyst
Article Abstract:
A novel method for the preparation of monosialoganglioside GM1 was developed based on a sialidase-producing (YF-2) bacterium isolated from seawater and gills of marine fish. The Pseudomonas sp. YF2 strain was identified from a synthetic medium containing crude bovine brain gangliosides. The synthetic reaction was characterized by the conversion of polysialogangliosides to GM1 by a sialidase catalyst that was produced by the YF-2 strain. Furthermore, the YF-2-based method can be utilized for the production of GM1 on an industrial scale.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Stereospecific biohydroxylations of protected carboxylic acids with Cunninghamella blakesleeana
Article Abstract:
Cunninghamella blakesleeana hydroxylates cycloalkylcarboxylic acids that have been protected against microbial transformation by conversion into 2-cycloalkyl-1,3-benzoxazoles. Preferential hydroxylation occurs for the carbon atoms trans to the benzoxazole group. The amounts of substrate hydrolyzed and product formed decrease with an increase in the ring size of the alkyl group. The hydroxylation of the 2-cycloalkyl-1,3-benzoxazoles produces low yield of alcohols and ketones.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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