Stoichiometric interpretation of Escherichia coli glucose catabolism under various oxygenation rates
Article Abstract:
A flux balance approach was used to derive optimal metabolic performance of Escherichia coli under varying oxygen constraints, and this technique uses linear optimization to find optimal metabolic flux patterns of cell growth. Derivations of optimal growth revealed that increased oxygen limitations caused the secretion of acetate, formate and ethano. By-product secretion rates increased linearly with the growth rate. Changes in metabolic pathway utilization were noticed with by-product secretion, and these changes resulted in variations in the various redox carriers.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Cultivation of Escherichia coli with mixtures of 3-phenylpropionic acid and glucose: steady-state growth kinetics
Article Abstract:
The cultivation of Escherichia coli with mixtures of 3-Phenylpropionic acid and glucose was examined to investigate the validity of the simple model of mixed-substrate growth. The model utilized a system that consisted of E. coli ML 30 growing with glucose and 3-phenylpropionic acid as the only sources of carbon and energy. Results indicate the need for further improvement of existing biodegradation-biotransformation processes for better understanding of microbial culture behavior with respect to mixed substrates.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Culturing enterohemorrhagic Escherichia coli in the presence and absence of glucose as a simple means of evaluating the acid tolerance of stationary-phase cells
Article Abstract:
Enterohemorrhagic Escherichia coli strains are acid resistant when cultured in tryptic soy broth (TSG) containing glucose, while there is a difference in the acid tolerance levels of cells cultured in TSG lacking glucose. Acid exposure causes acid injury followed by a loss of viability. The results of the study indicate that E. coli strains contain both pH-independent and pH-dependent stationary phase acid tolerant phenotypes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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