Structural, functional and mutational analysis of the pfr gene encoding a ferritin from Helicobacter pylori
Article Abstract:
The Helicobacter pylori bacterium causes certain upper gastrointestinal tract diseases. The function of the pfr gene encoding ferritin from H. pylori has been investigated using the Fur titration assay (FURTA) in Escherichia coli. The pfr region was found to be conserved amongst strains, and two transcriptional start sites were identified. The results suggested the involvement of the nonhaem-iron ferritin in the formation of iron-containing subcellular structures and contribution to metal resistance of H. pylori.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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Identification of a 29 kDa flagellar sheath protein in Helicobacter pylori using a murine monoclonal antibody
Article Abstract:
Immunogold-electron microscopy reveals that GF6, a murine monoclonal antibody to Helicobacter pylori, labels the membrane-like flagellar sheath specifically. Immunoblot-reveals the interaction of the antibody with a 29-kDa polypeptide and weak interaction with Vibrio cholerae and vibrio parahaemolyticus polypeptides of molecular mass 65 kDa. Immunoblotting and one- and two-dimensional electrophoretic analysis reveal that the antigen differs from the urease subunit ureA of similar molecular mass.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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A novel tRNA-associated locus (trl) from Helicobacter pylori is co-transcribed with tRNA(super Gly) and reveals genetic diversity
Article Abstract:
A study was conducted to analyze identify genes not expressed in all clinical isolates of Helicobacter pylori. The arbitrarily primed polymerase chain reaction (PCR) of RNA was utilized to determine a cDNA fragment of 187 bp. Nucleotide sequence information surround the tri locus were obtained using inverse PCR. Experimental results indicated that the locus is situated between two tRNA genes in H pylori. Findings also showed additional genetic diversity among these isolates.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
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