Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement
Article Abstract:
The 70S ribosome changes from a compact structure to a non-compact structure affecting the arrangement of proteins as revealed by cryo-electron microscopy density map real-space refinement analysis. The briges formed by proteins exhibit conformational changes upon a ratchet-like motion. Data indicate that the 70S ribosome of Escherichia coli presents two different functional states.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2003
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Structure of the mammalian mitochondrial ribosome reveals an expanded functional role for its component proteins
Article Abstract:
A three-dimensional cryo-electron microscopic analysis of the mammalian mitochondrial 55S ribosome carrying a tRNA at its P site show many proteins localized to new positions and the ribosome exhibits inter-subunit bridges consisting mainly proteins. Data show a gatelike structure at the mRNA entrance and a polypeptide exit tunnel.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2003
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Locking and unlocking of ribosomal motions
Article Abstract:
The rachet-like rotation of the 30S subunit with respect to the 50S ribosome subunit, during the ribosomal translocation, involves a 20 angstrom movement of the L1 stalk of the 50S subunit. Data indicate that the ribosomal motions requires deacylation of the P-site tRNA, which upon charging locks the ribosome.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2003
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