Suitability of PCR fingerprinting, infrequent-restriction-site PCR, and pulsed-field gel electrophoresis, combined with computerized gel analysis, in library typing of Salmonella enterica serovar enteritidis
Article Abstract:
Pulsed-field gel electrophoresis combined with computer analysis appears to be more suitable than PCR fingerprinting or infrequent-restriction-site PCR for typing Salmonella enterica serovar enteritidis. This was the conclusion of researchers who used 212 strains of the bacterium to create a library typing computerized system.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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Detection of a Salmonella enterica serovar California strain spreading in Spanish feed mills and genetic characterization with DNA microarrays
Article Abstract:
An epidemiological study on Salmonella isolated from raw plant based feed in Spanish meal was performed. Pulsed- field gel electrophoresis (PFGE) studies suggested genetic adaptation of the strain over time, while microarray analysis was used to further characterize the genetic repertoire present in this serovar.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
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Modification of kanamycin-esculin-azide agar to improve selectivity in the enumeration of fecal streptococci from water samples
Article Abstract:
The modified form of kanamycin-esculin-azide (KAA) agar effectively acts as an enumeration media for the determination of fecal streptococci from water samples. The substitution of kanamycin sulfate with oxolinic acid and an increase in the concentration of sodium azide modifies KAA agar to oxolinic acid-esculin-azide (OAA) agar. OAA agar is superior to KAA agar on the basis of selectivity, recovery efficiency, and specificity. OAA prevents the growth of background microbes. Membrane filtration of the water samples shows that OAA agar identifies almost 90% of fecal streptococci colonies.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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