Suppression of sorbitol dependence in a strain bearing a mutation in the SRB1/PSA1/VIG9 gene encoding GDP-mannose pyrophosphorylase by PDE2 overexpression suggest a role for the Ras/cAMP signal-transduction pathway in the control of yeast cell-wall biogenesis

Article Abstract:

The PSA1/VIG9 gene is the wild-type SRB1 gene in Saccharomyces cerevisiae, not the PDE2 gene. This conclusion supports the role of the Ras/cAMP pathway in cell-wall construction.

United Kingdom, Genetic aspects, Plant cell walls, Cell walls

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Cloning and sequencing of the Candida albicans homologue of SRB1/PSA1/VIG9, the essential gene encoding GDP-mannose pyrophosphorylase in Saccharomyces cerevisiae

Article Abstract:

The gene encoding the GDP-mannose pyrophosphorylase in Saccharomyces cerevisiae is essential. It has been independently isolated as a suppressor of alg1-PSA1 and dpm1 as SRB1. The cloning and molecular characterization of the SRB1.PSA1 homologue from C. albicans has been undertaken. It is shown to be unique in the C. albicans genome and it is suggested that it encodes a highly conserved protein similar to its s. cerevisiae counterpart.

Glycosylation, Candida albicans

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Down-regulation of the expression of PKC1 and SRB1/PSA1/VIG9, two genes involved in cell wall integrity in Saccharomyces cerevisiae, causes flocculation

Article Abstract:

A study was conducted to analyze the expression of the cell wall integrity determinants PKC1 and SRB1/PSA1/VIG9 of Saccharomyces cerevisiae under the influence of tightly regulated promoter pMET3. The strains were prepared in minimal medium while yeast total RNA was determined and separated on a denaturing agarose gel. Cultures of strains supporting pMET-3 regulated cassettes were grown at 250 rpm and 30 degrees C.

Analysis, Cells (Biology), Yeast, Yeast (Food product), Cells, Flocculation, Flocculation tests

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