Suppressors of a Saccharomyces cerevisiae pkc1 mutation identify alleles of the phosphatase gene PTC1 and of a novel gene encoding a putative basic leucine zipper protein

Article Abstract:

The influence of recombination mutants of the essential gene protein kinase C1 (PKC1) allele, pkc1-4, on the regulation of cell metabolism was investigated. The pkc1-4 allele was identified using colony sectoring assay. Two significant observations support that two separate Pkc1p-controlled pathways control regulation of cell wall metabolism and recombination in yeast. First, the addition of osmotically supportive agents to the medium suppressed the pkc1 growth defect but did not suppress the phenotypes conferred by the different pkc1 alleles. Second, mutation in MPK1, which encodes a MAPK cascade that regulates a pathway in yeast, did not affect recombination.

Protein kinases, Plant cell walls, Cell walls

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Multiple pathways for homologous recombination in Saccharomyces cervisiae

Article Abstract:

Examination of the requirement of genes in the RAD52 epistasis group of Saccharomyces cerevisiae for mitotic and meiotic recombination events by intrachromosomal inverted-repeat assay reveals the presence of at least three different RAD52-dependent recombination mechanisms in the yeast. A RAD51-dependent pathway involves most of the recombination events. Inverted-repeat recombination does not require the RAD1 gene, while in the absence of RAD51, RAD1 gene process spontaneous DNA lesions to generate recombination products.

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Stimulation of meiotic recombination in yeast by an ARS element

Article Abstract:

The effect of the replication origin ARS307 on meiotic recombination events was examined. Results indicate that ARS307 stimulates between one-half and two-thirds of recombination events in the hotspot region. Deletion of ARS307 reduces gene conversions and reciprocal crossovers in the region. It was found that an ARS element fragment of 200 bp is enough to induce recombination within the coldspot region. Furthermore, the element's orientation was found to influence the distribution of recombination events.

Analysis, Chromosome replication, Meiosis

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