Temperature-sensitive cdc7 mutations of Saccharomyces cerevisiae are suppressed by the DBF4 gene, which is required for the G(sub 1)/S cell cycle transition
Article Abstract:
A gene involved in the the initiation of chromosomal replication in Saccharomyces cerevisiae was isolated. Complementation studies identified the gene as DBF4, which codes for a protein of 695 residues, with a molecular mass of 80 kDa. The gene suppressesa temperature-sensitive cdc7-1 mutation.Further mutational analysis indicates that the products of these two genes interact in such a way as to allow the initiation of yeast chromosome replication, particulary at the G(sub 1) to S cell cycle transition.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
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Molecular genetic studies of the Cdc7 protein kinase and induced mutagenesis in yeast
Article Abstract:
An analysis of induced mutagenesis in Saccharomyces cerevisiae cdc7 mutants showed the mutants were hypomutable in one region and hypermutable in another. The relative mutability of all the cdc7 mutants, including two which were created by site-directed and insertional mutagenesis, was measured and correlated with the position of the mutation. The location of the temperature sensitive (ts) cdc7 alleles suggests some methods for predicting ts protein kinase mutations.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
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A conditional allele of the Saccharomyces cerevisiae HOP1 gene is suppressed by overexpression of two other meiosis-specific genes: RED1 and REC104
Article Abstract:
Two high copy plasmids that suppress the temperature-sensitive spore inviability phenotype in hop 1-628 was identified. Results showed that suppression is allele-specific and that the plasmids are necessary in meiosis. DNA sequence analysis indicated that the one of the high copy suppressors is RED1, a meiosis-specific gene. The other suppressor, which was mapped at the region on chromosome VIII 75kb distal to CDC12, was revealed to be REC 104.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1993
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