Teschoviruses as indicators of porcine fecal contamination of surface water
Article Abstract:
Research describes the development of a real-time reverse transcriptase coupled polymerase chain reaction method for the quantitative detection of pig teschovirus RNA. in contaminated water. Data show that the method is very specific, sensitive and detects 92 femtogram of viral RnA in one milliliter of water sample. Further, the method identifies the origin of the contamination unlike other methods.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
User Contributions:
Comment about this article or add new information about this topic:
Multiplex reverse transcription-PCR for simultaneous detection of Beet necrotic yellow vein virus, Beet soilborne virus and Beet virus Q and their vector polymyxa betae KESKIN on sugar beet
Article Abstract:
The detection threshold pf the multiplex reverse transcription-polymerase chain reaction assay for the three title viruses and their vector is up to 128 times greater than that of an enzyme-linked immunosorbent assay. Data indicate that Beet necrotic yellow vein virus is associated with one or two pomoviruses.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
User Contributions:
Comment about this article or add new information about this topic:
Multiplex quantitative real-time reverse transcriptase PCR for [F.sup.+]-specific RNA coliphages: A method for use in microbial source tracking
Article Abstract:
A culture-independent multiplex real-time reverse transcriptase-PCR (RT-PCR) protocol for the simultaneous quantification of all four subgroups of [F.sup.+]-specific RNA coliphages using novel primer sets and molecular beacons is presented.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Assessment of the ratio of pollen to seed flow in a cline for genetic variation in a quantitative trait. Reproductive processes in two oak (Quercus) contact zones with different levels of hybridization
- Abstracts: Seventeen complementation groups of mutations decreasing meitotic recombination in Schizosaccharomyces pombe. Meiotically induced rec7 and rec8 genes of Schizosaccharomyces pombe
- Abstracts: Effects of methylated, organic, and inorganic substrates on microbial consumption of dimethyl sulfide in estuarine waters
- Abstracts: A mechanism of cyclin D1 action encoded in the patterns of gene expression in human cancer. Schizosaccharomyces pombe Int6 and Ras homologs regulate cell division and mitotic fidelity via the proteasome
- Abstracts: PCR detection of virulence genes in Yersinia enterocolitica and Yersinia pseudotuberculosis and investigation of virulence gene distribution