The Bacillus stearothermophilus NUB36 surA gene encodes a thermophilic sucrase related to Bacillus subtilis SacA
Article Abstract:
The sucrase, encoded by the surA gene of Bacillus stearothermophilus, shows a sequence similarity with sucrose hydrolising enzymes of B. subtilis, Klebsiella pneumoniae and Vibrio alginolyticus. The enzyme's sucrose box, a sequence of nine amino acids, is important in the catalysis of sucrose into glucose and fructose. SurA shows a high degree of stability at room temperature, with an optimum temperature of 55 degree celsius for sucrose hydrolysis. The active form of the enzyme is a dimer and it is unable to operate during sucrose phosphate cleavage.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
bctA: a novel pBF4 gene necessary for conjugal transfer in Bacteroides spp
Article Abstract:
The genetic basis of pBF-4-mediated conjugal transfer in Bacteroides spp. was investigated. Recombinatorial mutagenesis using a suicide vector carrying fragments of the pBF4 plasmid was used to identify pBF4 genes governing conjugation. The identification of the first conjugation-specific gene of pBF4 was foreseen to provide the basis for further investigations of pBF4-mediated conjugation.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: The C. elegans spe-9 gene encodes a sperm transmembrane protein that contains EGF-like repeats and is required for fertilization
- Abstracts: Systemic lupus erythematosus. Histone deacetylase activity is required for full transcriptional repression by mSin3A
- Abstracts: The phage-like element PBSX and part of the skin element, which are resident at different locations on the Bacillus subtilis chromosome, are highly homologous
- Abstracts: A putative integrase gene defines the distal end of a large cluster of ToxR-regulated colonization genes in Vibrio cholerae