The bacterial flagellin gene as a biomarker for detection, population genetics and epidemiological analysis
Article Abstract:
The flagellin gene is responsible for the movement of a majority of bacterial species. The arrangement of flagella in the external surface body of a bacteria, its composition, gene sequence and forms, vary to identify the type of species they inhabit upon. Microbiological researches are conducted based on these diversities, particularly with the flagellin gene's domainal structure made of N- and C- terminal regions, making them ideal candidates for the PCR amplification. Furthermore, the diversity of motile bacteria qualify for the study of population genetics for a range of organisms.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Comparison of flagellin genes from clinical and environmental Pseudomonas aeruginosa isolates
Article Abstract:
The isolation of Pseudomonas aeruginosa strains obtained from an experimental mushroom-growing unit and other sources along with the analysis of the flagellin gene sequences have been undertaken using polymerase chain reaction restriction fragment length polymorphism analysis. P. aeruginosa is the most common pathogen associated with cystic fibrosis and is a major cause of hospital infections especially in immunocompromised patients. Results indicate that it is difficult to distinguish the environmental from the clinical types of P. aeruginosa.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
Flagellin gene and protein variation amongst clinical isolates of Pseudomonas aeruginosa
Article Abstract:
The flagellin gene is a highly useful genetic marker with many applications for studying genetic variation in closely related bacteria. PCR amplification and RFLP analysis of flagellin gene sequences from 64 clinical isolates of Pseudomonas aeruginosa comprised a strategy for differentiating between clinical isolates of P. aeruginosa. The technique proved to be quick, reproducible and applicable to all isolates.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Phylogenetics: Fisher's markers of admixture. Population genetics: DNAs from the European Neolithic. Allelic diversity of metallothionein in Orchesella cincta (L.): Traces of natural selection by environmental pollution
- Abstracts: Comparison of commercially available kits with standard methods for the detection of coliforms and Escherichia coli in foods
- Abstracts: Interchromosomal transfer of epigenetic states in Ascobolus: transfer of DNA methylation is mechanistically related to homologous recombination
- Abstracts: Requirement for the Candida albicans FAS2 gene for infection in a rat model of oropharyngeal candidiasis. Basis of cerulenin resistance of two strains of Candida albicans
- Abstracts: Neuronal target recognition. Pak functions downstream of dock to regulate photoreceptor axon guidance in Drosophila